Table 3. Results obtained from the set 2 of samples analyzed at NVI and IRL using three ELISA rapid tests and two western blot methods.

The O.D. represents the mean of duplicates /triplicates values obtained from each dilution sample by rapid tests. The values above the cut-off indicate positive sample and those below the cut-off indicate negative sample. Pos and Neg indicate positive and negative results by Western blot analyses.

ID sample	Dilution	HerdCheckBSE/Scrapie Antigen Test with Bovine conjugate, short protocol		TeSeE™ Sheep & Goat	TeSeE™ SAP	TeSeE™ Western Blot	SAF–Immunoblot
		NVI–Optical density	IRL–Optical density	Optical density	Optical density	Sha31	Sha31	6H4	9A2	L42	SAF84
Moose A	undiluted	3,382	3,317	0,176	0,017	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 2	3,505	3,314	0,158	0,014	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 4	3,5	3,307	0,072	0,015	Pos	Pos	Pos	Neg	Neg	Pos
	1 = 8	2,947	3,097	0,04	0,012	Pos	Pos	Neg	Neg	Neg	Pos
	1 = 16	2,164	2,6	0,047	0,013	Pos	Pos	Neg	Neg	Neg	Pos
	1 = 32	1,555	2,068	0,027	0,012	Pos	Pos	Neg	Neg	Neg	Pos
	1 = 64	1,011	1,039	0,021	0,012	Pos	Pos	Neg	Neg	Neg	Pos
	1 = 128	0,711	0,694	0,024	0,025	Pos	Pos	Neg	Neg	Neg	Neg
Moose B	undiluted	3,418	3,395	0,08	0,022	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 2	3,478	3,374	0,101	0,012	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 4	3,152	3,287	0,031	0,014	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 8	2,28	2,878	0,025	0,014	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 16	1,555	1,919	0,014	0,012	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 32	0,815	1,527	0,027	0,01	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 64	0,45	0,666	0,012	0,011	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 128	0,277	0,354	0,013	0,012	Pos	Pos	Neg	Neg	Neg	Neg
Moose C	undiluted	3,372	3,24	0,027	0,461	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 2	3,509	3,274	0,051	0,151	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 4	3,5	3,263	0,061	0.024	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 8	2,957	3,067	0,025	0,018	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 16	2,291	2,571	0,032	0,013	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 32	1,481	1,901	0,026	0,018	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 64	0,994	1,292	0,019	0,036	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 128	0,638	0,794	0,016	0,016	Pos	Pos	Neg	Neg	Neg	Neg
Moose D	undiluted	3,372	3,192	2.17	0,501	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 2	3,5	3,198	1,987	0,446	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 4	3,5	3,185	0,651	0,336	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 8	3,432	3,408	1,624	0,202	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 16	2,906	3,366	0.543	0,329	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 32	2,139	2,878	0,672	0,129	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 64	1,386	1,725	0,201	0,107	Pos	Pos	Pos	Pos	Pos	Pos
	1 = 128	0,949	1,076	0,118	0,053	Pos	Pos	Pos	Pos	Neg	Neg
Moose E	undiluted	0,627	0,332	0,02	0,012	Pos	Pos	Neg	Pos	Neg	Pos
	1 = 2	0,253	0,3	0,021	0,012	Pos	Pos	Neg	Pos	Neg	Pos
	1 = 4	0,137	0,196	0,013	0,011	Pos	Pos	Neg	Neg	Neg	Neg
	1 = 8	0,071	0,118	0,011	0,01	Pos	Neg	Neg	Neg	Neg	Neg
	1 = 16	0,044	0,079	0,011	0,011	Neg	Neg	Neg	Neg	Neg	Neg
	1 = 32	0,028	0,047	0,014	0,008	Neg	Neg	Neg	Neg	Neg	Neg
	1 = 64	0,029	0,042	0,017	0,009	Neg	Neg	Neg	Neg	Neg	Neg
	1 = 128	0,021	0,063	0,017	0,008	Neg	Neg	Neg	Neg	Neg	Neg
Cut-Off		0,168	0,191	0,151	0,116	//////	//////	//////	//////	//////	//////