Table 1. Summary of GABA_B-induced hyperpolarization of arcuate NPY neurons.

Baclofen Conc.	NPYG2WT neuron	NPYG2KO neuron
1 μM	−16.3 ± 3.0 mV (n = 7, 88%) Erev = −94.9 ± 6.1 mV	−13.1 ± 2.4 mV (n = 6, 67%, p = 0.431) Erev = −99.8 ± 11.1 mV
10 μM	−14.1 ± 1.9 mV (n = 12, 86%) Erev = −104.3 ± 5.3 mV	−20.9 ± 2.4 mV (n = 8, 100%, p = 0.035) Erev = −94.3 ± 2.0 mV
30 μM	−19.1 ± 2.0 mV (n = 12, 100%) Erev = −99.6 ± 4.8 mV	−20.8 ± 3.3 mV (n = 9, 100%, p = 0.641) Erev = −83.1 ± 3.8 mV
100 μM	−20.0 ± 2.0 mV (n = 14, 100%) Erev = −92.7 ± 3.1 mV	−26.7 ± 3.2 mV (n = 9, 100%, p = 0.075) Erev = −89.8 ± 3.7 mV

Changes of membrane potential are presented as mean ± SEM. Numbers in parentheses indicate the number of responsive cells, response rate, and p values (unpaired t test, NPY^G2WT neurons vs. NPY^G2KO neurons). E_rev = reversal potential. The individual numerical data for changes of membrane potential and reversal potential can be found in S8 Data.

NPY, neuropeptide Y.