Fig 3. p300 and CBP are important for supporting MCPyV transcription during infection.

(A) Whole cell lysates of HDFs transfected with siRNA targeting p300 (sip300), CBP (siCBP), or a scrambled control were collected at d3 and d7 post-transfection for Western blot analysis. (B) HDFs were transfected with siRNA against p300 (sip300), CBP (siCBP), or a scrambled control 24h prior to infection with 10⁸ viral genome equivalents of MCPyV. RT-qPCR analysis of viral mRNA expression in MCPyV-infected HDFs was performed on days 3 through 6 post-infection. Changes in LT and VP1 expression in the KD cells relative to the levels of viral transcription in control siRNA-transfected HDFs were calculated and normalized to cellular levels of GAPDH or actin mRNA as indicated. Error bars represent the standard deviation of three independent experiments. ****p<0.0001; ***p<0.001; **p<0.01; *p<0.05; ns = not significant.