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. 2023 Aug 30;19(8):e1011598. doi: 10.1371/journal.ppat.1011598

Fig 7. NF-κB p65 regulates MCPyV EP-driven transcription in a tightly controlled manner.

Fig 7

HEK293 cells were transfected with an MCPyV EP-luciferase reporter, a control reporter expressing Renilla luciferase, and the indicated amounts of a p65 expression plasmid. Cells were collected 24h after transfection for Western blot analysis (A), luciferase assay (B), or RT-qPCR analysis for IκB mRNA (C). Luciferase readings were normalized to the Renilla luciferase values for each sample. Changes in IκB mRNA level were normalized to cellular GAPDH mRNA. Error bars represent the standard deviation of three independent experiments. ****p<0.0001; ***p<0.001; **p<0.01; ns = not significant.