Three housekeeping proteins altered in diabetic human hearts. a Immunoblotting images of β-actin, calsequestrin (CSQ), and GAPDH in right atria (RA) of donors with diabetes mellitus (DM) compared to that of aged-matched controls (CTL); b immunoblotting images of β-actin, CSQ, and GAPDH in the left ventricle of donors with diabetes mellitus (DM) compared to that of aged-matched controls (CTL); c–e summarized data of β-actin (c), CSQ (d), and GAPDH (e) protein expression in right atria (left two bars) and left ventricle (right two bars) of diabetic hearts (DM) compared to that of aged-matched controls (CTL); f–h comparing summarized immunoblotting raw data normalized with total protein intensities from either PS-stained blot (two bars at far left) or CB-stained gel (two bars at far right) images. Presented data of the two bars at far left in f–h are the same as that of the two bars at far left in c–e. Data are represented as mean ± SEM. *p < 0.05; **p < 0.01. DM RA, n = 6; CTL RA, n = 4; DM LV, n = 6; CTL LV, n = 5