Extended Data Fig. 3. Unconditionally-generated designs are folded and thermostable.

A) Four 200 amino acid and fourteen 300 amino acid proteins were tested for expression and stability. 9/18 designs expressed, with a major peak at the expected elution volume. Blue: 300 amino acid proteins; Purple: 200 amino acid proteins. B) Colored AF2 predictions overlaid on gray design models (left), circular dichroism spectra at 25 °C (blue) and 95 °C (pink) (middle) and circular dichroism melt curves (right) for all 9 designs passing expression thresholds. In all cases, proteins remain well folded even at 95 °C. Note that data on 300aa_3 and 300aa_8 are duplicated from Fig. 2f, reproduced here for clarity.