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. 2023 Aug 16;620(7976):1054–1062. doi: 10.1038/s41586-023-06429-2

Fig. 5. RNAylation of the ribosome and phenotype of a ModB mutant T4 phage.

Fig. 5

a, Characterization of ModB substrate specificity. RNAylation of two ribosomal proteins (rS1 and rL2) in cell lysates and 70S ribosome assemblies (n = 3). b, Illustration of the RNAylated proteins rS1 and rL2 in the context of the 70S ribosome, based on the cryo-electron microscopy structure of the hibernating 70S E. coli ribosome (PDB: 6H4N)51. c–e, Characterization of the T4 ModB R73A, G74A mutant phenotype, showing the burst size (c), E. coli lysis (d) and phage adsorption (e) of WT T4 phages and T4 ModB(R73A, G74A) (n = 3 biologically independent replicates for each). Data points with error bars represent mean ± s.d. Grey dotted boxes indicate time points used for assessing statistical significance in the case of burst size (c, 140 min after infection; two-sided Student’s t-test, P = 0.0015 at Psignif < 0.05) and phage adsorption (e, 8 min after infection; t-test, two-sided, P = 0.029 at Psignif < 0.05) but indicate the delayed lysis without a statistical test in d. Statistical tests are shown in Supplementary Fig. 4.

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