Fig. 4. Fcn B transported by BLM-induced AMs exosomes contributed to lung epithelial cells autophagy and ferroptosis.

A The particle size of exosomes was detected by NTA. B The morphology of exosomes was characterized by TEM. C The expressions of CD9, CD63, and CD81 in exosomes were characterized by Western blot. D Exosome uptake experiment. E, F The mRNA expression of Fcn B in lung epithelial cells was detected by RT-qPCR. G, H The expressions of LC3II/LC3I, Beclin1, ATG7, and p62 in lung epithelial cells were detected by Western blot. I The concentrations of MDA, GSH, and Fe²⁺ in lung epithelial cells were detected by biochemical kits. J ROS levels in lung epithelial cells were determined by flow cytometry. K The death rate of lung epithelial cells was determined by an LDH assay kit. L, M The expressions of GPX4, SLC7A11, FTH1, FTL1, and NCOA4 in lung epithelial cells were detected by Western blot. The experiment was repeated three times. The data were presented as the mean ± SD. *BLM^si-NC vs. Control, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ^#BLM^si-Fcnb vs. BLM^si-NC, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001; ^$BLM^si-Fcnb +Exo^oe-NC vs. BLM^si-Fcnb, ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, ^$$$$P < 0.0001; & BLM^si-Fcnb +Exo^oe-Fcnb vs. BLM^si-Fcnb +Exo^oe-NC, ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001, ^&&&&P < 0.0001.