Fig. 6. Inhibition of autophagy alleviated lung epithelial cells ferroptosis promoted by Fcn B from BLM-induced AMs exosomes through the cGAS-STING pathway.

A Mitochondrial damage and autophagosomes were detected by TEM (The yellow arrow represented normal mitochondria, the red arrow represented damaged mitochondria, and the blue arrow represented autophagosomes). B The expressions of LC3II/LC3I, Beclin1, ATG7, and p62 in lung epithelial cells were detected by Western blot. C, D The concentrations of MDA, GSH, and Fe²⁺ in lung epithelial cells were detected by biochemical kits. E ROS levels in lung epithelial cells were determined by flow cytometry. F The death rate of lung epithelial cells was determined by an LDH assay kit. G, H The expressions of GPX4, SLC7A11, FTH1, FTL1, and NCOA4 in lung epithelial cells were detected by Western blot. The experiment was repeated three times. The data were presented as the mean ± SD. ^#BLM^si-cGAS or 3-MA vs. BLM^si-NC, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001; ^$BLM^si-cGAS+Exo^oe-NC vs. BLM^si-cGAS, ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, ^$$$$P < 0.0001; ^@BLM^si-cGAS+Exo^oe-Fcnb vs. BLM^si-cGAS+Exo^oe-NC, ^@P < 0.05, ^@@P < 0.01, ^@@@P < 0.001, ^@@@@P < 0.0001; & BLM^si-cGAS+Exo^oe-Fcnb+3-MA vs. BLM^si-cGAS+Exo^oe-Fcnb, ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001, ^@@@@P < 0.0001.