Fig. 8. Fcn B transported from AMs exosomes facilitated ferroptosis in BLM-induced mice.

A Mitochondrial damage and autophagosomes were detected by TEM (The red arrow represented damaged mitochondria). B The expressions of LC3II/LC3I, Beclin1, ATG7, and p62 in lung were detected by Western blot. C, D The concentrations of MDA, GSH, and Fe²⁺ in lung and serum were detected by biochemical kits. E ROS levels in lung were determined by flow cytometry. F, G The expression of GPX4, SLC7A11, FTH1, FTL1, and NCOA4 in lung were detected by Western blot. H The expressions of cGAS, STING, and p-STING in lung were detected by Western blot. The experiment was repeated three times (n = 10 mice/group). The data were presented as the mean ± SD. *WT+BLM vs. WT, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ^$WT+BLM+Exo^oe-NC vs. WT+BLM, ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001, ^$$$$P < 0.0001; & WT+BLM+Exo^oe-Fcnb vs. WT+BLM+Exo^oe-NC, ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001, ^&&&&P < 0.0001; ^#Fcnb^-/-+BLM vs. WT+BLM, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001; @ Fcnb^-/-+BLM+Exo^oe-NC vs. Fcnb^-/-+BLM, ^@P < 0.05, ^@@P < 0.01, ^@@@P < 0.001, ^@@@@P < 0.0001; ^ΔFcnb^-/-+BLM+Exo^oe-Fcnb vs. Fcnb^-/-+BLM+Exo^oe-NC, ^ΔP < 0.05, ^ΔΔP < 0.01, ^ΔΔΔP < 0.001, ^ΔΔΔΔP < 0.0001.