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. 2023 Aug 30;13(9):e1393. doi: 10.1002/ctm2.1393

FIGURE 5.

FIGURE 5

Motif analysis of small eccDNA junctions in cancer plasma and multi‐cancer diagnostic value of small eccDNAs. (A) Genomic distributions of small eccDNAs in cancer plasma and cancer tissues. UTR, untranslated region; TTS, transcription start site; LINEs, long interspersed nuclear elements; SINEs, short interspersed nuclear elements; LTR, long terminal repeat. * There are significant differences. (B) Nucleotide motif sequences flanking the start and end positions of small eccDNA junctions in cancer plasma. (C) The multi‐cancer diagnostic value in plasma of the proportion of small eccDNAs originated from some genes (all AUC > .9 and P < .05). ZNF423, zinc finger protein 423; SHISA9, shisa family member 9; TMEM132B, transmembrane protein 132B; GRID2, glutamate ionotropic receptor delta type subunit 2; STARD13, StAR related lipid transfer domain containing 13; DMD, dystrophin; DLC1, DLC1 Rho GTPase activating protein; LMCD1‐AS1, LMCD1 antisense RNA 1; RPS6KA2, ribosomal protein S6 kinase A2; SLC4A4, solute carrier family 4 member 4; ATP8A2, ATPase phospholipid transporting 8A2; AUC, the area under the ROC curve. (D) The multi‐cancer diagnostic value in tissues of the proportion of small eccDNAs originated from ALK and ETV6 (P < 0.05). ALK, ALK receptor tyrosine kinase; ETV6, ETS variant transcription factor 6. (E) Great multi‐cancer diagnostic value in tissues of the combination of the proportion of small eccDNAs originated from some specific genes and CEA (all AUC > .8 and P < .05). ERG, ETS transcription factor ERG; CEA, carcinoembryonic antigen; SNORD161, small nucleolar RNA, C/D box 161; LUZP2, leucine zipper protein 2. (F–G) Great multi‐cancer diagnostic value in plasma of the combination of the proportion of small eccDNAs originated from some specific genes and CEA/CA19‐9 (all AUC ≥ .95 and P < .05). CA19‐9, carbohydrate antigen 19‐9.