Fig. 2.

GJ protein Zpg intrinsically controls GSC maintenance and early GSC progeny differentiation. (A) Confocal images show Inx2 and Zpg colocalization. STED images indicate that Inx2 on IGS cells and Zpg on GSC progeny form a heterologous GJ channel. (Scale bars, 0.2 µm.) (B and C) Germline-specific zpgKD decreases GSC and increases CB-like cells compared to the control. Cap cells, GSCs, and CBs are highlighted by ovals, arrows, and arrowheads, respectively. (C) quantification results. n = number of germaria. (Scale bars, 10 µm.) Student’s t test: ***P ≤ 0.001. (D and E) Germline-specific zpgKD decreases γ-H2Av expression (broken rectangle) in meiotic 16-cell cysts in region 2a compared to the control. (E) quantification results. n = number of germaria. (Scale bars, 10 µm.) (F and G) Germline-specific zpgKD increases the incidence of two pro-oocytes (arrowheads) cysts in stage-1 egg chambers. (G) quantification results. n = number of germaria. (Scale bars, 10 µm.)