Fig. 3.
IGS-expressed cAMP is transported to GSCs and their early progeny via GJs. (A and B) Single section confocal images showing that cAMP is highly enriched in IGS cells and weakly expressed in GSCs and their early progeny. 13C06ts-driven Inx2 knockdown and nos-Geneswitch-Gal4-driven germline zpg knockdown increase cAMP levels in IGS cells and decrease cAMP levels in GSCs and their early progeny. With signal enhancement, cAMP expression can still be detected in GSCs and their progeny. (B) quantification results. (Scale bars, 10 µm, 2 µm.) Student’s t test: ***P ≤ 0.001. (C and D) Live imaging of freshly dissected germaria expressing the nos-Nano-lantern-cAMP reporter. 13C06ts-driven Inx2 knockdown decreases the cAMP reporter activity in germ cells. (D) quantification results. (Scale bars, 10 µm.) Student’s t test: ***P ≤ 0.001. (E and F) Live imaging of freshly dissected germaria expressing the cAMP reporter with or without the treatment of the CBX inhibitor. After a 15-min treatment, cAMP levels are significantly reduced in GSCs and their progeny. (F) quantification results. (Scale bars, 10 µm.) Student’s t test: ***P ≤ 0.001.