Fig. 6.

NLRP3 deficiency reduces neutrophil chemotaxis toward a laser-induced liver burn injury. (A) Representative intravital microscopy images acquired every 30 s for a period of 150 min following laser-induced liver burn injury. Neutrophil migration toward the burn was tracked during this period. Neutrophils are highlighted by yellow arrows, directionality of the arrow depicts the direction of neutrophil migration in the next frame. We observed an increase in blood vessel dextran leakage in the livers of NLRP3^+/+ mice following injury, seen as an increasing area of green fluorescence outside the liver microvasculature. (B) Visualization of neutrophil tracking during the 150-min follow-up period. Arrows depict the start and end point of the neutrophil during the 150 min of follow-up. (C) Quantification of the fraction of neutrophils that migrated toward the site of injury over the 150-min follow-up period by a blinded investigator, presented as the percentage of neutrophils from the total number of neutrophils inside the field of view during the follow-up period. Unpaired t test was used for statistical analysis of two groups (P < 0.05 *, n = 3).