| Antibodies |
|
| Anti-human CD31-A Alexa Fluor® 488 (clone 390) |
BioLegend |
Cat#102414; RRID: AB_493408 |
| Anti-human IgG-Alexa Fluor® 647 |
Thermo Fisher Scientific |
Cat#A-21445; RRID: AB_2535862 |
| Human PfCSP mAbs |
Wang et al., 20208 Kisalu et al. 20189
|
N/A |
| Control chicken PfCSP mAb (6F8) |
Nacer et al., 202234
|
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Recombinant PfCSP constructs with truncated repeat region |
Wang et al., 20208
|
GenBank Accession No. MT891160, MT891178
|
| IMALGENE 1000® (ketamine) |
Boehringer Ingelheim |
N/A |
| Rompun 2% solution (xylazine) |
Bayer |
N/A |
| Avertin |
Alfa Aesar |
Cat#A18706 |
| Propidium Iodide |
Invitrogen |
Cat#P3566 |
| Matrigel® matrix |
Corning |
Cat#356237 |
| 16% Formaldehyde |
Thermo Fisher Scientific |
Cat#28906 |
|
| Critical commercial assays |
|
| rProtein A Sepharose |
Millipore Sigma |
Cat#GE17-1279-03 |
| Expi293 Expression System Kit |
Thermo Fisher Scientific |
Cat#A14635 |
| FreeStyle 293 Expression System |
Thermo Fisher Scientific |
Cat#K900001 |
| Protein G chromatography cartridge |
Thermo Fisher Scientific |
Cat#89927 |
|
| Experimental models: Cell lines |
|
| Human: Expi293 cells |
Thermo Fisher Scientific |
Cat#A14527 |
| Human: 293D cells |
Thermo Fisher Scientific |
Cat#R79007
|
|
| Experimental models: Organisms/strains |
|
| Mouse: RjOrl:SWISS |
Janvier Laboratories |
Cat#SN-SWISS-F |
| Mouse: C57BL/6JRj |
Janvier Laboratories |
Cat#SC-C57J-F |
| Mouse: C57BL/6 |
Charles River Laboratories |
Cat#027 |
| Mosquitoes: Anopheles stephensi (SDA 500 strain) |
CEPIA |
N/A |
| Sporozoite: P. berghei expressing PfCSP and GFP |
Wang et al., 20208
|
N/A |
| Sporozoite: P. berghei expressing mCherry |
Burda et al., 201535
|
N/A |
| Sporozoite: P. berghei expressing PfCSP, GFP, and luciferase |
Flores-Garcia et al., 201936
|
N/A |
|
| Software and algorithms |
|
| GraphPad Prism 9 |
GraphPad |
http://www.graphpad.com/; RRID:SCR_002798 |
| RStudio |
RStudio |
https://rstudio.com/; RRID:SCR_000432 |
| R language |
CRAN |
http://cran.r-project.org/; RRID:SCR_003005 |
| Tidyverse |
R package |
https://cran.r-project.org/web/packages/tidyverse/index.htm; RRID:SCR_019186 |
| Corrplot |
R package |
https://github.com/taiyun/corrplot; RRID:SCR_023081 |
| Fiji |
Schindelin et al., 201237
|
https://fiji.sc; RRID:SCR_002285 |
| MTrack2 |
Fiji Plug-in |
https://valelab4.ucsf.edu/~nstuurman/IJplugins/MTrack2.html
|
| MTrackJ |
Fiji Plug-in |
https://CRAN.R-project.org/package=mdftracks
|
| CytExpert 2.0 |
Beckman Coulter |
https://www.beckman.fr/flow-cytometry/instruments/cytoflex/software; RRID:SCR_017217 |
| Octet Software, version 7.0 |
FortéBio |
http://www.fortebio.com
|
|
| Other |
|
| CytoFLEX S flow cytometer |
Beckman Coulter |
N/A |
| Axio Observer Z.1 |
Zeiss |
N/A |
| Spinning-disk confocal system Ultra View ERS |
Perkin Elmer |
N/A |
| VP-ITC microcalorimeter |
Malvern Panalytical |
N/A |
| Octet HTX N/A |
ForteBio |
N/A |
