Table 3.
Results of the N-alkylamides HPLC–MS-DAD quantification in A. oleracea n-hexane and methanol extracts
| N-Alkylamide | n-Hexane extract | Methanol extract | |||||
|---|---|---|---|---|---|---|---|
| Concentration (g/100 g DE1)2 ± SD3 |
RSD%4 | Absolute amount of N-alkylamides extracted (g/100 g DB)5 |
Mean concentration (g/100 g DE) ± SD |
RSD% | Absolute amount of N-alkylamides extracted (g/100 g DB)e |
||
| A1 | (2Z)-N-Isobutyl-2-nonene-6,8-diynamide | 0.4 ± 0.1 | 16.7 | < 0.1 | 0.1 ± 0.0 | 0.0 | < 0.1 |
| A2 | (2E)-N-Isobutyl-2-undecene-8,10-diynamide | 0.3 ± 0.0 | 0.0 | < 0.1 | 0.1 ± 0.0 | 0.0 | < 0.1 |
| A3 | (2E,6Z,8E)-N-Isobutyl-2,6,8-decatrienamide (spilanthol) | 24.3 ± 1.3 | 4.0 | 1.3 | 7.6 ± 0.3 | 3.4 | 1.9 |
| A4 | (2E,7Z)-N-Isobutyl-2,7-decadienamide | 0.2 ± 0.0 | 0.0 | < 0.1 | 0.1 ± 0.0 | 0.0 | < 0.1 |
| A5 | (2E)-N-(2-Methylbutyl)-2-undecene-8,10-diynamide | ||||||
| A6 | (2E,6Z,8E)-N-(2-Methylbutyl)-2,6,8-decatrienamide | 1.7 ± 0.3 | 13.0 | 0.1 | 0.5 ± 0.0 | 0.0 | < 0.1 |
1DE, dry extract
2Average concentration (g/100 g DE) represents the average concentration of N-alkylamides found in each DE and it is the mean of two independent analyses
3SD, standard deviation
4RSD%, relative SD
5Amount of N-alkylamides (g/100 g) extracted from dry biomass