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. Author manuscript; available in PMC: 2024 May 12.
Published in final edited form as: Sci Immunol. 2023 May 5;8(83):eadh3455. doi: 10.1126/sciimmunol.adh3455

Fig. 3: Elevation of immune cytokines in myopericarditis and identification of immune cell subsets.

Fig. 3:

(A) Scatter plots of selected cytokines in patients (n = 9) and healthy younger vaccinated controls (YVC, n = 6). Statistical significance was determined using the unpaired two-sided Wilcoxon rank-sum test with Benjamini-Hochberg FDR correction for multiple comparisons. All additional serum proteins assayed are shown in Fig. S3 (B) Biplot of all serum proteins assayed (84 total) between patients and healthy YVC (see related principal component analysis (PCA) in Fig. S4, A and B). (C) Schematic diagram showing the cohort studied in subsequent scRNA-seq analyses, including pediatric male healthy donors (HD, n = 4), healthy early-young vaccinated controls (E-YVC, n = 4), acute myopericarditis patients (n = 4), and matched patients at follow-up/recovery (n = 3). (D) UMAP visualization of immune cell subsets identified from the cohort in (C), with additional four CITE-seq multisystem inflammatory syndrome in children (MIS-C) after SARS-CoV-2 samples included to refine cluster annotation using surface proteins. Cell subset abbreviations: activated (act), class-switched (sw), class-unswitched (u-sw), memory (mem), natural killer (NK), natural killer T (NKT; CD3+ CD161+ KLRF1+), regulatory T (Treg), interferon-stimulated gene (ISG), cytotoxic T lymphocyte (CTL), proliferating (prolif), mucosal-associated invariant T (MAIT), terminally differentiated effector memory CD45RA+ T (TEMRA), non-classical (non-class), intermediate (int), classical (class), monocyte (mono), conventional dendritic cell (cDC), plasmacytoid dendritic cell (pDC), migratory (mig), hematopoietic stem and progenitor cell (HSPC).