Skip to main content
. 2023 Aug 18;4(3):102522. doi: 10.1016/j.xpro.2023.102522

Figure 3.

Figure 3

Examples of DAPI-stained cells from both release assays

(A and B) Representative images of DAPI-stained cells from (A) G1 (MGY2150) and (B) Cdc20 (MGY1293) release assays. Cells were imaged with 12 z-planes at 0.5 μm intervals using DIC and DAPI settings, and maximum z-projection images are shown for DAPI, and a single focal plane for DIC. Cells scored in the G1 release assay (A) are grouped into three categories based on cell and DNA morphology: G1/metaphase - small to large budded with 1 DAPI focus, anaphase - large budded with 2 DAPI foci, and anaphase re-bud / schmooing cells with 1 DAPI focus (seen in later time points). In the Cdc20 release assay (B), cells are grouped into three similar categories: single cells - unbudded with 1 DAPI focus, metaphase - small to large budded with 1 DAPI focus, and anaphase - large budded with 2 DAPI foci. If a large budded cell has a DAPI signal that is dumbbell shaped but still connected, it is counted as a metaphase cell in both the G1 and Cdc20 release assays. Scale bars = 2 μm.