| Chemicals, peptides, and recombinant proteins |
|
| Methyl cellulose (viscosity: 4,000 cP) |
Sigma Life Sciences |
Product #M0512 |
| Taxol (paclitaxel, >99.5%) |
TSZ CHEM |
Cat# ROS036 |
| L-Methionine, 98+% |
Acro Organics |
Code: 166161000 |
| Yeast nitrogen base without amino acids |
BD Difco™ |
Ref# 291940 |
| Alpha-factor mating pheromone (10 mM in 0.1 M sodium acetate pH 5.2) |
Zymo Research |
Cat# Y1001 |
| Bacto peptone |
BD Bacto™ Peptone |
Ref# 211705 |
| Synthetic Complete Media |
Sunrise Science Products |
Cat# 1300-030 |
| SC-MET Media |
Sunrise Science Products |
Cat # 1343-030 |
| Propidium iodide (0.5 mg/mL solution) |
Roche |
11819000 |
|
| Experimental models: Organisms/strains |
|
|
S. cerevisiae: Strain background S288C; See Table S1
|
N/A |
S288C |