Table 7.
Percentage of Each of the 20 Classes That Expressed 12 Discriminating Markers
| Class | NOS | ChAT | 5-HT | SP | ENK | CGRP | SOM | Calb | Calret | NF200 | VIP | NPY |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AIN1 | 0.0%a | 100.0% | 0.0%a | 100.0%a | 100.0%a | 0.0% | 1.9% | 14.8% | 0.0% | 90.7% | 1.9% | 0.0% |
| AIN2 | 0.0%a | 100.0% | 0.0%a | 0.0%a | 100.0%a | 0.0% | 2.8% | 100.0%a | 26.8% | 91.5% | 1.4% | 0.0% |
| AIN3 | 0.0%a | 100.0% | 0.0%a | 0.0%a | 100.0%a | 0.0% | 1.6% | 0.0%a | 0.0% | 86.5% | 0.8% | 0.8% |
| DIN1 | 100.0%a | 95.1% | 100.0%a | 12.2% | 4.9% | 36.6% | 7.3% | 53.7% | 12.2% | 92.7% | 80.5% | 7.3% |
| DIN2 | 0.0%a | 100.0%a | 100.0%a | 26.7% | 6.7% | 53.3% | 6.7% | 53.3% | 93.3% | 86.7% | 13.3% | 6.7% |
| DIN3 | 100.0%a | 100.0%a | 0.0%a | 5.5% | 1.6% | 2.4% | 0.8% | 56.7% | 11.8% | 100.0%a | 79.5% | 7.1% |
| DIN4 | 100.0%a | 100.0%a | 0.0%a | 1.6% | 3.2% | 0.0% | 1.6% | 8.1% | 14.5% | 0.0%a | 29.0% | 11.3% |
| DIN5 | 0.0%a | 100.0%a | 0.0%a | 0.0% | 0.0%a | 1.9% | 0.0% | 100.0%a | 84.6% | 98.1% | 100.0%a | 0.0% |
| DIN6 | 0.0%a | 100.0%a | 0.0%a | 13.6% | 0.0%a | 0.0% | 4.5% | 0.0%a | 4.5% | 72.7% | 100.0%a | 4.5% |
| EMN1 | 0.0% | 100.0% | 0.0%a | 1.7% | 0.0% | 1.7% | 1.1% | 100.0%a | 12.6% | 100.0%a | 0.0% | 0.6% |
| EMN2 | 0.0% | 100.0% | 0.0%a | 0.0% | 0.0% | 0.5% | 2.6% | 0.0% | 0.0% | 100.0%a | 0.0% | 0.0% |
| EMN3 | 0.0% | 100.0% | 0.0%a | 4.8% | 0.0% | 1.2% | 0.0% | 100.0%a | 10.8% | 0.0% | 0.0% | 1.2% |
| EMN4 | 0.0% | 100.0% | 0.0%a | 1.8% | 0.0% | 0.3% | 0.3% | 0.0% | 2.4% | 0.0% | 0.0% | 0.6% |
| IMN1 | 100.0%a | 0.0%a | 0.0%a | 0.8% | 0.4% | 0.0% | 0.4% | 30.6% | 3.0% | 100.0%a | 100.0%a | 20.4% |
| IMN2 | 100.0%a | 0.0%a | 0.0%a | 1.8% | 0.0% | 0.0% | 0.0% | 16.4% | 0.9% | 100.0%a | 0.0%a | 22.7% |
| IMN3 | 100.0%a | 0.0%a | 0.0%a | 0.5% | 0.0% | 0.0% | 0.3% | 0.8% | 2.3% | 0.0%a | 100.0%a | 12.0% |
| IMN4 | 100.0%a | 0.0%a | 0.0%a | 0.6% | 0.0% | 0.0% | 0.0% | 3.2% | 2.9% | 0.0%a | 0.0%a | 6.1% |
| Misc | 0.0%a | 0.0%a | 0.0% | 2.1% | 4.2% | 0.0% | 0.0% | 35.4% | 10.4% | 25.0% | 10.4% | 0.0% |
| SN1 | 0.0% | 100.0% | 26.7% | 88.0% | 0.0% | 20.0% | 12.0% | 73.3% | 100.0%a | 100.0%a | 1.3% | 1.3% |
| SN2 | 0.0% | 100.0% | 0.0% | 100.0% | 0.0% | 0.0% | 31.8% | 18.2% | 0.0%a | 100.0%a | 0.0% | 0.0% |
| All cells | 50.5% | 56.4% | 2.9% | 7.9% | 10.1% | 1.8% | 1.7% | 26.5% | 9.7% | 51.7% | 34.4% | 6.6% |
Twelve immunohistochemical markers expressed by 20 identified classes of neurons in human colon. Percentages refer to the proportion of cells of that class which expressed each of the markers.
5-HT, 5-hydroxytryptamine; AIN, ascending interneuron; Calb, calbindin; Calret, calretinin; CGRP, calcitonin gene-related peptide; ChAT, choline acetyltransferase; DIN, descending interneuron; EMN, excitatory motor neuron; ENK, leu enkephalin; IMN, inhibitory motor neuron; Misc, miscellaneous; NF200, neurofilament 200 kD; NOS, neuronal nitric oxide synthase; NPY, neuropeptide Y; NS, nonsignificant; SN, sensory neuron; SOM, somatostatin; SP, substance P; VIP, vasoactive intestinal polypeptide.
Values of 0% or 100% that were set as discriminating markers during the divisive hierarchical clustering process (see Materials and Methods).