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. 2022 Nov 30;28(5):511–528. doi: 10.1007/s12192-022-01316-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Tomato bZIP28 transactivation activity. A Schematic representation of the domains of bZIP28. Numbers indicate amino acids. NTAD, N-terminal activation domain region; bZIP, basic leucine zipper; TMD, transmembrane domain; S1P, metalloprotease recognition site. B GUS reporter assay on PCNX1::GUS reporter of the indicated bZIP28p mutants expressed in tomato mesophyll protoplasts. Values are average of normalized GUS activity relative to mock control (n = 3). Error bars are ± SD and different letters indicate significant differences based on ANOVA and Duncan’s multiple range test (p < 0.05). Immunoblot analysis of HA-tagged bZIP28p proteins expressed in protoplasts for the GUS reporter assay. LBRC, large subunit of RuBisCO. C Subcellular localization of bZIP28pΔ1-162-GFP or GFP alone in tomato protoplasts. Images are representative of many independent cells that showed the same result. OL, overlay of GFP and autofluorescence; BF, brightfield. D Transactivation activity of bZIP28p using GUS reporter assay on promoters (1 kb) of selected tomato genes. Values are average of normalized GUS activity relative to mock control (n = 3). Error bars are ± SEM and asterisks indicate significant differences based on Student’s t-test (*p < 0.05, ***p < 0.001)