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. 2022 Nov 30;28(5):511–528. doi: 10.1007/s12192-022-01316-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Transactivation activity of HsfA1a, HsfA2, bZIP60s, and bZIP28p on GUS reporters of ER chaperones and ER-UPR genes. A Cis-elements corresponding to HSF or bZIP28/bZIP60 binding sites in the promoters (approximately 1000 bp upstream of TSS) of ER chaperones and UPR genes. More detailed information can be found in Supplementary Dataset 1. B–I GUS reporter assays on different promoters in tomato protoplasts transformed with the indicated reporter and combinations of HsfA1a, HsfA2, bZIP28p, and/or bZIP60s. GUS activity values are the average of independent transformations from independent experiments (n = 6–9) and have been normalized against the mock control (onefold). Error bars are ± SEM. Different letters denote statistically significant difference between samples, based on one-way ANOVA test and Duncan’s multiple range post hoc test (p < 0.05)