Fig. 7.

Transactivation activity of BIP3 GUS reporter by HSFs and bZIPs and TUN-triggered activity of HsfA1a. A GUS reporter assays of PBIP3::GUS in tomato protoplasts co-transformed with combinations of HsfA1a, HsfA2, bZIP28p, and/or bZIP60s. The amount of plasmid DNA carrying the transcription factor in micrograms used to transform 50,000 protoplasts is indicated below. GUS activity values are the average of independent protoplast transformations (n = 3). Error bars are ± SEM. Different letters denote statistically significant difference between samples, based on one-way ANOVA test and Duncan’s multiple range post hoc test (p < 0.05). B GUS reporter assay using either PHsp17-CI or CNX2 as reporters in protoplasts treated with the indicated amounts of TUN. GUS activity values are the average of independent transformations from independent experiments (n = 6–9) and have been normalized against the mock control (onefold) individually for each set. Error bars are ± SEM. Asterisks indicate statistically significant difference between samples, based on one-way ANOVA test and Duncan’s multiple range post hoc test (p < 0.05) performed independently for each set