Table 1.

Change in miRNA levels with low and high EGFR, ERK, and PTEN protein levels.

Subgroups		Baseline			3 months of 5-FU therapy			6 months of 5-FU therapy			P-value
miRNA223-3p
ERK	≤ 0.92	6.22	5.65	6.39	5.52	4.24	6.12	5.82	5.39	6.39	0.247
	> 0.92	6.07	5.77	6.76	5.2	4.65	5.82	6.32	5.34	7.08	0.031
P-value		0.121			0.895			0.303
PTEN	≤ 1407.85	6.28	5.94	6.57	5.52	3.51	6.41	5.82	5.09	6.39	0.344
	> 1407.85	6.07	5.65	6.39	5.38	4.65	5.55	6.32	5.52	7.08	0.011
P-value		0.119			0.885			0.228
EGFR	≤ 6.53	6.28	5.74	6.57	5.49	4.24	6.41	6	5.39	6.89	0.247
	> 6.53	6.19	5.81	6.55	5.2	4.65	5.82	6.05	5.34	6.71	0.038
P-value		0.12			0.728			0.915
miRNA20a-5p
ERK	≤ 0.92	4.74	4.19	6.01	4.29	3.47	5.13	4.62	4.05	5.43	0.766
	> 0.92	4.66	4.34	5.22	4.42	3.6	4.74	5.07	4.52	6.21	0.155
P-value		0.121			0.057			0.707			0.364
PTEN	≤ 1407.85	4.52	4.1	6.01	4.42	3.47	5.13	4.62	4.13	5.61	0.936
	> 1407.85	4.87	4.59	5.77	4.3	3.6	4.74	5.07	4.05	6.02	0.085
P-value		0.119			0.036			0.675			0.812
EGFR	≤ 6.53	4.74	4.19	6.01	4.29	3.47	5.13	4.62	4.05	5.43	0.766
	> 6.53	4.66	4.34	5.22	4.42	3.6	4.74	5.07	4.52	6.21	0.155
P-value		0.12			0.057			0.707			0.364
miRNA19a-3p
ERK	≤ 0.92	3.53	2.23	4.45	2.78	1.57	3.52	3.63	3.29	4.63	0.165
	> 0.92	4.18	3.54	5	2.58	1.76	3.72	4.26	3.1	4.98	0.007
P-value		0.121			0.018			0.757			0.669
PTEN	≤ 1407.85	3.53	2.82	4.6	2.81	1.43	3.92	3.59	3.27	4.6	0.42
	> 1407.85	3.77	2.91	4.35	2.58	2.06	3.72	4.26	3.34	4.98	0.005
P-value		0.119			0.101						0.73
EGFR	≤ 6.53	3.53	2.84	4.6	2.81	1.55	3.92	3.63	3.29	4.63	0.42
	> 6.53	3.87	2.91	4.35	2.47	1.8	3.17	3.94	3.24	4.8	0.005
P-value		0.12			0.09			0.696			0.768
miRNA17-5p
ERK	≤ 0.92	3.3	3.09	3.89	3.37	2.72	4.19	3.49	3.09	4.38	0.819
	> 0.92	4.15	3.61	4.89	3.31	2.47	3.76	3.95	2.9	5.06	0.041
P-value		0.121			0.009			0.646			0.448
PTEN	≤ 1407.85	3.62	3.11	4.89	3.16	2.49	4.19	3.46	2.97	4.38	0.936
	> 1407.85	3.7	3.29	4.66	3.41	2.66	3.59	3.95	3.51	5.06	0.127
P-value		0.119			0.062			0.744			0.212
EGFR	≤ 6.53	3.35	3.11	4.89	3.37	2.49	4.19	3.49	3.09	4.58	1
	> 6.53	3.74	3.48	4.76	3.31	2.66	3.59	3.95	2.97	4.89	0.074
P-value		0.12			0.043			0.679			0.647
miRNA7-5p
ERK	≤ 0.92	1.23	− 0.17	1.73	0.61	− 0.23	1.38	0.55	0.44	1.48	1
	> 0.92	1.25	0.94	2.16	0.42	− 0.34	1.01	1.31	0.84	2.46	0.011
P-value		0.121			< 0.001			0.435			0.277
PTEN	≤ 1407.85	1.45	− 0.09	2.2	0.13	− 0.45	1.38	1.17	0.44	1.48	0.549
	> 1407.85	1.21	0.92	1.91	0.47	0.06	1.09	1.08	0.74	2.48	0.155
P-value		0.119			0.002			0.628			0.49
EGFR	≤ 6.53	1.41	− 0.09	2.2	0.47	− 0.34	1.36	1.19	0.44	1.64	0.766
	> 6.53	1.23	0.92	1.91	0.43	− 0.01	1.01	1.08	0.74	2.21	0.038
P-value		0.12			0.001			0.595			0.736

Data presented as medians and IQR of miRNAs level. Significant P-values ≤ 0.01 are displayed in bold- italic font.

IQR interquartile range.