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. 1998 Apr;36(4):1074–1080. doi: 10.1128/jcm.36.4.1074-1080.1998

FIG. 2.

FIG. 2

Influence of synthetic epitope flagellin antigen on free and IC-derived L antibodies to B. burgdorferi. Serum samples from patients containing IC-derived antibody (precipitated with PEG and resuspended in PEG) assayed with biotinylated whole-cell sonicate (Bb-bio) alone (a), serum samples containing IC-derived antibody assayed with biotinylated whole-cell sonicate (Bb-bio) plus biotinylated flagellin synthetic epitope-BSA conjugate (Fla-bio) (b), serum samples containing free (untreated) antibody assayed with biotinylated whole-cell sonicate (Bb-bio) alone (c), and serum samples containing free (untreated) antibody assayed with biotinylated whole-cell sonicate (Bb-bio) plus biotinylated flagellin synthetic epitope-BSA conjugate (Fla-bio) (d) as the antigen source were used to detect LD-specific IgM antibodies in M-capture ELISA. Results are expressed as an index value, as defined in Materials and Methods.