FIG. 3.

M-capture ELISA used to detect LD-specific IgM antibodies in patients with multiple lesions, patients with single lesions, and negative control patients. Serum samples from patients containing IC-derived antibody (precipitated with PEG and resuspended in PEG) assayed with biotinylated whole-cell sonicate (Bb-bio) alone (a), serum samples containing IC assayed with biotinylated whole-cell sonicate (Bb-bio) plus biotinylated flagellin synthetic epitope-BSA conjugate (Fla-bio) (b), and free (untreated) serum samples containing free (untreated) antibody assayed with biotinylated whole-cell sonicate (Bb-bio) alone (c) as the antigen source were tested. Results are expressed as an index value, as defined in Materials and Methods.