TABLE 1.
Type of LD and patient | Culture for B. burgdorferi | M-capture ELISA result with the following:
|
Result of IFA for IgM | Result of EIA (recombinanti dot blot) for IgM | Result of immunoblotting for IgM | Result of polyvalent EIA | ||
---|---|---|---|---|---|---|---|---|
Sonicate + flagellin (IC) | Sonicate (IC-derived antibody) | Sonicate (free antibody) | ||||||
Secondary | ||||||||
MC-2 | POS | POS | POS | POS | POS | POS | NEG | POS |
MC-10 | POS | POS | POS | POS | POS | POS | NEG | POS |
MC-23 | POS | POS | NEG | NEG | POS | POS | POS | POS |
MC-28 | POS | POS | POS | ND | POS | POS | POS | POS |
MC-20 | POS | POS | POS | ND | POS | POS | POS | POS |
MC-3 | POS | POS | POS | ND | POS | NEG | NEG | NEG |
MC-14 | POS | POS | POS | ND | POS | POS | POS | POS |
MC-9 | POS | POS | POS | ND | POS | POS | NEG | POS |
MC-41 | POS | POS | POS | NEG | POS | EQUIV | NEG | POS |
Primary | ||||||||
MC-11 | POS | EQUIV | EQUIV | NEG | POS | NEG | NEG | NEG |
MC-35 | POS | POS | POS | POS | NEG | EQUIV | POS | NEG |
MC-48 | POS | POS | POS | POS | POS | NEG | NEG | NEG |
MC-55 | POS | POS | POS | POS | POS | EQUIV | POS | NEG |
MC-4 | POS | POS | POS | ND | NEG | NEG | NEG | NEG |
MC-17 | POS | POS | NEG | ND | POS | NEG | NEG | POS |
MC-36 | POS | POS | POS | ND | NEG | POS | POS | POS |
MC-72 | POS | POS | POS | ND | EQUIV | NEG | POS | POS |
MC-25 | POS | POS | EQUIV | POS | POS | NEG | NEG | NEG |
MC-24 | POS | NEG | NEG | NEG | POS | NEG | NEG | NEG |
MC-8 | POS | NEG | NEG | ND | NEG | NEG | NEG | NEG |
Although results are presented for the M-capture ELISA done in three ways, the direct comparison to other tests for LD should be done with the serum sample containing IC-derived antibody and assayed with the biotinylated whole-cell sonicate (Bb-bio) plus the biotinylated flagellin synthetic epitope-BSA carrier (Fla-bio) as the antigen source. The other Lyme tests for LD are described in Materials and Methods. POS, positive; NEG, negative; EQUIV, equivocal; ND, not determined.