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. 2023 Aug 21;120(35):e2301457120. doi: 10.1073/pnas.2301457120

Fig. 2.

Fig. 2.

The C-terminal region strongly enhances LLPS of CLIP-170. (A) Secondary structure of CLIP-170 (1 to 1,438), H2 (1 to 481), and H1 (1 to 350) drawn to scale, based on (20, 21, 50). (B) Representative confocal images of purified GFP-H1, GFP-H2, GFP-H2-tail, and GFP-FL-CLIP each at 200 nM in the absence (Top) or presence (Bottom) of 2% PEG. (Scale bar: 20 µm.) (C) Condensate surface coverage of the three constructs at indicated PEG concentrations. Mean with SD from three independent experiments with a total of 27 fields of view per condition. Statistics: two-tailed Student’s t test. (D) Droplet size (area) of GFP-H2 (200 nM) and GFP-FL-CLIP (200 nM). Mean with SD from three independent experiments with a total of 27 fields of view. Statistics: two-tailed Student’s t test. (E) Size distribution of GFP-FL-CLIP (200 nM) and GFP-H2 (200 nM) droplets in the absence of PEG. The graph shows average size distribution from three independent experiments with a total of 27 fields of view.