Schematic representation of the GE 16S rDNA, showing the relative locations of PCR primers used for the primary (ge3a and ge10r) and nested (ge9f and ge2) amplification reactions. The orientation of each primer is indicated by an arrowhead, and the sizes of the primary and nested products are shown. Scale, 16S rDNA base numbering. Small rectangle, region where sequence differences between the HGE agent, E. equi, and the GE variant were noted.