FIG. 5.

Sensitivity testing of the GE PCR assays. (A) Products of the primary assay using 10-fold serial dilutions of the positive control (pFC4) as templates. Lanes 1 through 10 correspond to template copy numbers ranging from 1.93 × 10⁷ (lane 1) to 0.0193 (lane 10). The expected size of the primary product (932 bp) is indicated. (B) Products of nested PCR using 1 μl of the corresponding primary product as the template, with the expected size of the nested PCR product (546 bp) indicated. Lanes M are molecular weight standards and show the HaeIII digest of phage φX174 DNA. Lanes C, negative controls representing the background human genome DNA as the template without the addition of the plasmid-cloned ehrlichia 16S rDNA.