Figure 2.

Efficiency of nr2e3 shRNA transfection on the migration, invasion and colony-formation of MCF7 cells. Evaluation of the effectiveness of nr2e3-targetting shRNAs using (A) reverse transcription-quantitative PCR and (B) western blotting, with GAPDH used as a control. (C) Representative images and (D) evaluation of the migratory ability using Transwell assays. The migratory cells were observed using an Olympus CKX53 inverted microscope at x200 magnification. Scale bar, 100 µm. (E) Evaluation of the migratory ability was examined by wound healing experiments. (F) Evaluation of the colony-formation ability. ^**P<0.01 and ^***P<0.001. C, control; NR2E3, nuclear receptor subfamily 2 group E member 3; ns, no significance; shRNA or sh, short hairpin RNA.