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. 2023 Aug 22;26(4):474. doi: 10.3892/etm.2023.12173

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of nr2e3 silencing on the expression of EMT markers, stem cell-related transcription factors and the proportion of CD44⁺CD24^-/low cells. Detection of the mRNA and protein expression levels of EMT markers and stem cell-related transcription factors using (A) reverse transcription-quantitative PCR and (B) western blotting, respectively, with GAPDH used as the endogenous control. (C) Proportion of CD44⁺CD24^-/low subgroup cells was analyzed using flow cytometry. (D) Semi-quantification of the expression ratio from (C). ^*P<0.05, ^**P<0.01 and ^***P<0.001. C, control; EMT, epithelial-mesenchymal transition; NR2E3, nuclear receptor subfamily 2 group E member 3; sh, short hairpin RNA; klf4, Krüppel-like factor 4.