Skip to main content
. 2023 Aug 22;26(4):474. doi: 10.3892/etm.2023.12173

Figure 4.

Figure 4

Nr2e3 knockdown decreases the expression level of nr2c2. (A) Correlation analysis between the mRNA expression levels between nr2e3 and nr2c2 in ER+ breast cancer tissues. Data were downloaded from TCGA database. (B) Nr2c2 expression was investigated in ER+ breast tumor samples compared with normal specimens from TCGA database. Expression levels of nr2c2 (C) mRNA and (D) protein were detected in nr2e3-silenced MCF7 and shNC-transfected cells using reverse transcription-quantitative PCR and western blotting, respectively, with GAPDH used as the control. ***P<0.001. (E) Protein expression levels of LSD1 and H3K4me2 were detected in NR2E3-depleted MCF7 and shNC cells using western blotting. GAPDH served as the endogenous control. BC, breast cancer; ER, estrogen receptor; LSD1, lysine-specific histone demethylase 1A; NR2C2, nuclear receptor subfamily 2 group C member 2; NR2E3, nuclear receptor subfamily 2 group E member 3; TCGA, The Cancer Genome Atlas.