Table II.
Primers used for reverse transcription-quantitative PCR.
| Gene (accession number) | Primer sequence (5' to 3') |
|---|---|
| Nr2e3 | F: GATCCTGAGCACGTAGAGGC |
| (NM_016346.4) | R: GCAATTTCCCAAACCTCACGG |
| Nr2c2 | F: GGCGCCAAATCCTGAGGTAA |
| (NM_003298.5) | R: GGTGAGGCTACAGCAGAGTC |
| Nanog | F: TCCTCCTCTTCCTCTATACTAAC |
| (NM_024865.4) | R: CCCACAAATCACAGGCATAG |
| Klf4 | F: ATCTCGGCCAATTTGGGGTT |
| (NM_004235.6) | R: CCAGGTGGCTGCCTCATTA |
| Oct4 | F: ATCGAGAACCGAGTGAGA |
| (NM_002701.6) | R: ACACTCGGACCACATCCTT |
| Sox2 | F: GGGAAATGGGAGGGGTGCAAAAGAGG |
| (NM_003106.4) | R: TTGCGTGAGTGTGGATGGGATTGGTGT |
| E-cadherin | F: CCTCCAGAGTTTACTGCCATGAC |
| (NM_001792.5) | R: GTAGGATCTCCGCCACTGATTC |
| N-cadherin | F: GGCGCCACCTGGAGAGA |
| (NM_004360.5) | R: TGTCGACCGGTGCAATCTT |
| Vimentin | F: TACAGGAAGCTGCTGGAAGG |
| (NM_003380.5) | R: ACCAGAGGGAGTGAATCCAG |
| GAPDH | F: GGAGCGAGATCCCTCCAAAAT |
| (NM_002046.7) | R: GGCTGTTGTCATACTTCTCATGG |
Nr2e3, nuclear receptor subfamily 2 group E member 3; Nr2c2, nuclear receptor subfamily 2 group C member 2; Nanog, nanog homeobox; Klf4, Krüppel-like factor 4; Oct4, POU class 5 homeobox 1; Sox2, SRY-box transcription factor 2; E-cadherin, cadherin 1; N-cadherin, cadherin 2. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward primer sequence; R, Reverse primer sequence.