Figure 2.

BGN deficiency inhibited cancer cell migration and MC38 cell allograft growth through enteric neurons

(A) The mouse enteric neurons were clustered to 7 clusters by tSNE.

(B) Visualization of BGN expression in mouse enteric neurons clusters by tSNE.

(C) BGN was expressed in mouse enteric neurons clusters.

(D and E) Representative image of ink propulsion distance in BGN ^(-/0) and WT mice and BGN deficiency reduced intestinal propulsion rate (n = 4 mice/group).

(F and G) Representative images of mouse enteric neurons of TUBB3 staining, by Immunofluorescence. BGN deficiency reduced the length of neurite (n = 3 mice/group).

(H) The model of transwell co-culture of cancer cell and enteric neurons (Created with Biorender.com).

(I and J) Representative image presenting transwell co-culture of MC-38 cells and the enteric neurons from BGN ^(-/0) and WT mice. The number of MC-38 cells migration was decreased in co-culture with BGN ^(-/0) mice enteric neurons (n = 3 biological replicates/group).

(K) Allograft mouse model (Created with Biorender.com).

(L) Representative image of tumors.

(M) BGN ^(-/0) mice enteric neurons reduced the tumor size (n = 11–14 mice/group).

(N) BGN ^(-/0) mice enteric neurons reduced the tumor weight (n = 11–14 mice/group).

Data are representative or cumulative results of at least two independent experiments (D–G, J and L–M). Data are presented as mean ± S.E.M. ∗ indicates WT + MC38 group VS. MC38 group, ^# indicates WT + MC38 group VS. BGN ^(-/0) +MC38 group, ∗p < 0.05, ∗∗p < 0.01. ∗∗∗p < 0.001.