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. 1998 Apr;36(4):1096–1100. doi: 10.1128/jcm.36.4.1096-1100.1998

FIG. 3.

FIG. 3

HS-causing type B P. multocida-specific PCR assay. This figure illustrates fragments specifically amplified by PCR from type B P. multocida organisms that cause hemorrhagic septicemia by means of the primers KTSP61 and KTT72. It can be seen that only P. multocida B:2, B:5, and B:2,5 produced amplification products. This gel shows a negative control (lane 1), P. multocida strain VP161, serotype A:1 (lane 2), VP21, A:3 (lane 3), VP17, A:4 (lane 4), P1511, B:1 (lane 5), 0332, B:2 (lane 6), VP164, B:5 (lane 7), VP145, B:2,5 (lane 8), P5226, B:3,4 (lane 9), P5325, B:4 (lane 10), 0349, D (lane 11), VP170, D:1 (lane 12), 0350, E (lane 13), P4218, F:3 (lane 14), and a 100-bp DNA marker (Promega) (lane M). Samples were electrophoresed at 2 V/cm for 2 h in a 2% agarose gel (1× TAE), stained with ethidium bromide, visualized by UV illumination, and photographed.