Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 31;42:223. doi: 10.1186/s13046-023-02793-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Oxidative stress is mostly responsible for the cytotoxic effect of the combination on MOLM-13 cells. A ROS levels were quantified in MOLM-13 cells by flow cytometry, upon the incorporation of the oxidation-sensitive dye CMH₂-DCFDA, 48 h after RBA treatment (one-way ANOVA). B TEM analysis focused on mitochondria of MOLM-13 cells, 24 h after treatment; areas delimited in dashed squares in the upper panels are magnified in the lower panels. C MOLM-13 cells mitochondrial membrane depolarization, measured by flow cytometry as increased green/red fluorescence ratio of the JC-1 dye, 48 h after treatments. On the left are shown representative flow cytometry plots, on the right the histogram reports average values of the green/red MFI ratio. D Confocal microscopy images of MOLM-13 cells stained with an anti-NRF-2 antibody. E NRF-2 expression in MOLM-13 cells, 24h after treatment, measured by flow cytometry (unpaired Student’s T test). F MOLM-13 cell death was measured by PI exclusion assay, 72 h after treatment with RBA, in the absence or in the presence of 20mM N-acetyl-cysteine (left, two-way ANOVA). G ROS measurement, in cells treated with or without NAC, for 48 h (right, two-way ANOVA)