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. 2023 Aug 17;14:1252344. doi: 10.3389/fmicb.2023.1252344

Table 2.

New putative T6SS effectors and cognate immunity proteins encoded in SPI-6 of Salmonella enterica.

T6SS effector genes Cognate T6SS immunity protein genes
ORF(s) Size (aa) Serotype-strain Variable region Predicted activity/domain ORF(s) TM or signal peptide/domain
Effectors targeting peptidoglycan
Unannotated ORF 32 S. Bareilly RSE03 1 Peptidoglycan hydrolase (Amidase)/L-Ala, D-Glu endopeptidase ELZ70_17800 Signal peptide (Sec/SPI)/No
S. Bredeney CVM24358 HFS03_00580
S. Daytona NCTC7102 NCTC7102_04795
S. Florida NCTC6480 NCTC6480_03851
S. Give NCTC5778 NCTC5778_03432
S. India SA20085604 Unannotated ORF
S. Mikawasima RSE13 Unannotated ORF
S. Paratyphi A ATCC9150 SPA_RS12640
S. Poona NCTC4840 NCTC4840_03690
S. enterica LHST_2018 Unannotated ORF
S. enterica NCTC7404 NCTC7404_03579
S. enterica NCTC7411 NCTC7411_03668
S. enterica NCTC7831 NCTC7831_03115
S. enterica NCTC8272 NCTC8272_03056
S. Sanjuan NCTC7406 NCTC7406_04092
S. Schwarzengrund CMV19633 SESA_RS02015
S. Senftenberg ATCC 43845 SEES3845_018760
S. Typhi CT18 STY_RS01380
NCTC7406_04082 122 S. Sanjuan NCTC7406 2 Peptidoglycan hydrolase (L,D transpeptidase)/Pgp2 NCTC7406_04081 Signal peptide (Sec/SPI)/No
G9X22_18260 279 S. Adjame 353,868 2 Peptidoglycan hydrolase (Amidase)/TseH-like Unannotated ORF 2 TM/DUF4229
NCTC5778_03416 S. Give NCTC5778 NCTC5778_03415
LFZ16_04210 S. India SA20085604 LFZ16_04215
SESA_RS02090 S. Schwarzengrund CMV19633 Unannotated ORF
SESEF3709_03438 243 S. enterica SESen3709 2 Peptidoglycan hydrolase (Amidase)/Reprolysin_4 SESEF3709_03437 Signal peptide (Sec/SPI)/No
EOS97_RS15095 Salmonella sp. SSDFZ54 EOS97_RS15100 No/No
SEES3845_018655 S. Senftenberg ATCC 43845 SEES3845_018650 No/No
CS349_18880 S. Tennessee CFSAN070645 CS349_18875 No/No
NCTC7411_03656 243 S. enterica NCTC7411 2 Peptidoglycan hydrolase (Amidase)/Peptidase_M64 NCTC7411_03655 1 TM/No
NCTC7831_03138 S. enterica NCTC7831 NCTC7831_03139 1 TM/No
NCTC7406_04078 S. Sanjuan NCTC7406 NCTC7406_04077 1 TM/No
Effectors targeting nucleic acids
Unannotated ORF 149 S. Kedougo Sal162 3 RNase and DNase/RhsA-Ntox47-Tox-HNH-EHHH Unannotated ORF No/Imm50
CS349_18795 S. Tennessee CFSAN070645 CS349_18790
NCTC7836_04182 970 S. enterica NCTC7836 3 DNase/RhsA-PDEEXK NCTC7836_04181 No/No
NCTC7836_04194 616 S. enterica NCTC7836 3 DNase/RhsA-Tox-HNH-EHHH NCTC7836_04193 No/No
NCTC8272_03019 589 S. enterica NCTC8272 NCTC8272_03018 No/No
SESEF3709_03428 592 S. enterica SESen3709 SESEF3709_03427 No/Imm50
G9X22_18245 1,501 S. Adjame 353,868 3 DNase/PAAR-RhsA-Tox-HNH-EHHH G9X22_18240 No/Imm50
CFSAN002050_RS06455 1,499 S. Cubana CFSAN002050 CFSAN002050_RS06460 No/No
HFQ57_17525 S. Havana CVM20761 HFQ57_17520 No/No
SEES3845_018630 S. Senftenberg ATCC 43845 Unannotated ORF No/No
HFQ45_19990 1,377 S. Anatum CVM20746 3 DNase/PAAR-RhsA-HNHc HFQ45_19995 No/SMI1_KNR4
NCTC5778_03412 1,382 S. Give NCTC5778 NCTC5778_03411 No/SMI1_KNR4
E4T58_01505 1,580 S. Infantis L41 E4T58_01510 No/No
SPA_RS12520 1,570 S. Paratyphi A ATCC9150 SPA_RS12515 No/No
NCTC7831_03143 1,382 S. enterica NCTC7831 NCTC7831_03144 No/SMI1_KNR4
SEES3845_018590 1,377 S. Senftenberg ATCC 43845 SEES3845_018585 No/SMI1_KNR4
NCTC7102_04762 1,044 S. Daytona NCTC7102 3 DNase/RhsA-WHH NCTC7102_04761 No/SMI1_KNR4
SESEF3709_03422 1,575 S. enterica SESen3709 3 DNase/PAAR-RhsA-WHH SESEF3709_03421 No/SMI1_KNR4
ELZ70_17690 1,354 S. Bareilly RSE03 3 DNase/PAAR-RhsA-AHH ELZ70_17685 No/No
HFQ57_17490 1,368 S. Havana CVM20761 HFQ57_17485
HI825_06260 1,317 S. enterica LHST_2018 HI825_06265
STY_RS01485 1,354 S. Typhi CT18 STY_RS01490
HF553_RS18710 1,374 Salmonella sp. SCFS4 3 DNase/PAAR-RhsA-GIY-YIG HF553_RS18705 No/CdiI
HU143_RS17590 Salmonella sp. SJTUF14076 HU143_RS17585
IVP14_RS17960 Salmonella sp. SJTUF14146 IVP14_RS17955
IVP15_RS18860 Salmonella sp. SJTUF14152 IVP15_RS18855
IVP16_RS17665 Salmonella sp. SJTUF14154 IVP16_RS17660
IVP17_RS17925 Salmonella sp. SJTUF14170 IVP17_RS17920
IVP18_RS17920 Salmonella sp. SJTUF14178 IVP18_RS17915
HFQ45_20000 174 S. Anatum CVM20746 3 RNase/RhsA-DUF4329 HFQ45_20005 No/CdiI
ELZ68_18315 267 S. Stanleyville RSE01 ELZ68_18310 No/No
SCH_RS26875 1,593 S. Choleraesuis SC-B67 3 RNase/PAAR-RhsA-DUF4329 SCH_RS01475 No/CdiI
SPC_RS25995 S. Paratyphi C RSK4594 SPC_RS01475
NCTC13175_03561 352 S. Goldcoast NCTC13175 3 RNase/RhsA-Ribonuclease/Microbial Rnase NCTC13175_03560 No/Barstar
Unannotated ORF 1,564 S. Derby Sa64 3 RNase/PAAR-RhsA-Ribonuclease/Microbial Rnase EIC79_17405 No/Barstar
SEBLO3795_03484 1,564 S. enterica SEHaa3795 SEBLO3795_03483
EOS98_RS24920 1,560 Salmonella sp. SSDFZ69 EOS98_RS17735
HLB37_13055 1,560 S. Kedougo Sal162 3 RNase/PAAR-RhsA-EndoU_bacteria HLB37_13060 No/MafI
SEHA_RS26915 102 S. Heidelberg SL476 3 RNase/CdiA SEHA_RS02130 No/Imm42
IA1_RS24740 S. Thompson RM6836 IA1_RS01635
HI825_06280 106 S. enterica LHST_2018 3 Deaminase/Tox-Deaminase HI825_06285 No/SUKH_5
STY_RS01505 86 S. Typhi CT18 STY_RS01510
Effectors targeting translation machinery
DYN42_004080 943 S. London CVM N17S347 3 ADP-ribosyltransferase/RhsA-Tox-ART-HYD1 DYN42_004085 No/No
NCTC8271_04564 194 S. enterica NCTC8271 Unannotated ORF
NCTC5741_00975 943 S. enterica NCTC5741 NCTC5741_00976
SESEF3709_03418 402 S. enterica SESen3709 SESEF3709_03417
IA1_RS01605 959 S. Thompson RM6836 IA1_RS01610
NCTC4840_03667 1,566 S. Poona NCTC4840 3 ADP-ribosyltransferase/PAAR-RhsA-Tox-ART-HYD1 NCTC4840_03666 No/No