TABLE 4.
Comparison of fast growth between phcA mutants and WT strains on amino acids, organic acids issued from the Krebs Cycle, and sugars and other discriminating metabolites as carbon sources a
| Strain | Psi07 | CFBP2957 | UW551 | GMI1000 |
|---|---|---|---|---|
| L-aspartic acid | 2 | 2 | 2 | 2 |
| L-glutamic acid | 2 | 2 | 2 | 2 |
| L-glutamine | 2 | 2 | 2 | 2 |
| L-asparagine | 2 | 2 | 2 | 2 |
| Fumaric acid | 2 | 2 | 2 | 2 |
| L-malic acid | 2 | 2 | 2 | 2 |
| Pyruvic acid | 2 | 2 | 2 | 2 |
| α-D-glucose | 1 | 2 | 2 | 2 |
| Citric acid | 1 | 2 | 2 | 2 |
| D-saccharic acid | 1 | 2 | 2 | 2 |
| D-trehalose | 1 | 2 | 0 | 2 |
| α-Ketoglutaric acid | 1 | 2 | 2 | 1 |
| Succinic acid | 1 | 1 | 2 | 1 |
| L-proline | 1 | 1 | 2 | 1 |
| L-histidine | 1 | 1 | 2 | 1 |
| Pectin | 1 | 2 | 1 | 1 |
| L-alanine | 1 | 1 | 1 | 1 |
| Sucrose | 0 | 1 | 2 | 0 |
| L-threonine | 0 | 1 | 2 | 1 |
| Acetic acid | 1 | 1 | 0 | 1 |
| D-galactose | 1 | 1 | 0 | 1 |
| D-fructose | 1 | 0 | 1 | 0 |
| L-Serine | 1 | 0 | 1 | 1 |
| D-gluconic acid | 1 | 1 | 1 | 1 |
| m-Inositol | 1 | 2 | 0 | 1 |
| Butyric acid | 2 | 1 | 0 | 1 |
| Glycerol | 1 | 1 | 2 | 1 |
a
2, both phcA mutant and WT strains grow fast on the substrate; 1, only phcA mutant grows fast on the substrate; 0, neither the WT nor the phcA mutant grows fast on the substrate. The trophic preferences were assessed using Biolog phenotype microplates PM1 and PM2-A and an in-house script, which detect automatically if there is fast growth (see Materials and Methods for details). The rest of the substrates are available in File S5 at https://github.com/cbaroukh/rssc-metabolic-networks.