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. 2023 Aug 7;26(9):107549. doi: 10.1016/j.isci.2023.107549

Figure 1.

Figure 1

Detection of vaccine-derived spike protein mRNA in human placental explants and four human cell lines using qRT-PCR

(A) Vaccine messenger RNA (mRNA) standard curves. Standard curves for mRNA-1273 and BNT162b2 vaccines were generated to calculate vaccine-derived spike mRNA concentrations in human placental explants and cells. The limit of detection (LOD) level was set according to the standard curve (0.05 pg/μL, mRNA-1237 CT = 23.2, BNT162b2 CT = 24.6).

(B) Vaccine mRNA detected in human placental explants and four different types of cell lines exposed to mRNA-1273 or BNT162B2 at 0.1 μg/mL or 1 μg/mL for 0.5 h or 4 h. Results were calculated by extrapolating CT values based on the derived standard curves for each mRNA vaccine. Black dotted line represents the LOD level of 0.05 pg/μL. Gray box represents the area under LOD. All data represent 2 independent experiments. CT, Cycle threshold.