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. 2023 Aug 7;26(9):107549. doi: 10.1016/j.isci.2023.107549

Figure 2.

Figure 2

Lack of detection of COVID mRNA vaccine via in situ hybridization in human placental explants

Chorionic villi explants derived from second (n = 2) or third trimester (n = 2) human placentas were incubated with 0.1 μg/mL (not shown) or 1 μg/mL mRNA-1237 or BNT162B2 vaccines. After 0.5 h or 4 h, tissues were fixed, paraffin-embedded, sectioned and probed for mRNA vaccine using RNAscope in situ hybridization.

(A and B) Positive and (B) negative controls for RNAscope detection of mRNA vaccine. Peptidylprolyl isomerase B (PPIB) was used as a positive control. Pink dots corresponding to PPIB mRNA can be observed within the chorionic villi at 20X and 40X. DapB was used as a negative control. No signal was detected at 20 or 40X.

(C) In situ detection of mRNA vaccine in vaccine-exposed explants. No signal was evident in explants incubated with either vaccine at any of the two time points.