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. 2023 Aug 30;21:330. doi: 10.1186/s12916-023-03040-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Activation and cytokines releasing of CAR-T cells co-cultured with NKYS. a Activation markers (CD69, CD25 and HLA-DR) expression on CD4 + and CD8 + subgroups of T cells were detected by FACS. b Statistical results of activation markers expression on T cells. ΔPercentage is equal to the difference between the proportion of positive subpopulation of T cells in the treatment group and blank group. c Cytokines released by CAR-T cells and control T cells were detected using the CBA Kit. Δpg/ml refers to the difference between cytokine concentration in the treatment and blank groups. A value of p < 0.05 was considered statistically significant. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001