Fig. 2.

Pharmacokinetics of subcutaneous LEN in pigtail macaques. Longitudinal plasma LEN concentrations measured by liquid chromatography mass spectrometry (LC-MS) in (a) naïve pigtail macaques that received two doses of LEN at 15 mg/kg (N = 3; blue diamonds) or 50 mg/kg (N = 3; black circles) 6 weeks apart and in (b) three pigtail macaques in the LEN PrEP study group that each received a single subcutaneous injection of LEN at 25 mg/kg and were intravenously challenged with stHIV-A19 30 days later. In each plot, the bottom horizontal red dashed line represents the assay lower limit of quantification (LLOQ; 0.1 nM) and the top horizontal black dashed line represents the plasma protein binding-adjusted EC₉₅ (PA-EC₉₅; 1.46 nM) for LEN against stHIV-A19 in pigtail macaque peripheral blood mononuclear cells.