Fig. 4.

Cell-associated viral DNA following stHIV-A19 challenge. Cell-associated viral DNA (CA-vDNA) levels in PBMC for (a) vehicle control, (b) TDF/FTC/DTG control PrEP, and (c) LEN PrEP group animals prior to and following intravenous stHIV-A19 challenge. The timing of vehicle, LEN, and stHIV-A19 injections are indicated by arrows. The grey shaded region in (b) indicates a 7-day period of daily TDF/FTC/DTG administration, beginning three days prior to stHIV-A19 challenge. Determined vDNA levels were normalized based on input diploid genome cell equivalents assayed for each sample determined by the duplex quantification of the copy number of a host cell gene within the same sample extraction. Symbols are plotted at the threshold sensitivity limit for that sample based on the number of cell equivalents assayed. Horizontal dashed lines indicate the highest threshold assay quantification limit (based on the lowest cell input for the assay) across all analysed samples (15 vDNA copies/10⁶ cell equivalents). Plot symbols below the quantification limit represent specimens in which no vDNA were detected.