Fig. 5.

Experimental depletion of CD8+ cells in LEN PrEP animals. Animals in the LEN PrEP group received a single subcutaneous injection of the rhesusized anti-CD8 antibody MT807R1 (50 mg/kg) at the indicated time point, after LEN plasma concentrations declined below the lower limit of quantitation (LLOQ) in each of the three animals. (a) Longitudinal plasma LEN concentrations measured by LC-MS. The bottom horizontal red dashed line represents the assay LLOQ for LEN (0.1 nM). The top horizontal dashed black line represents the PA-EC₉₅ (1.46 nM) for LEN against stHIV-A19 in pigtail macaque cells. (b) Longitudinal absolute CD8+ T cell counts in whole blood determined by flow cytometry. (c) Longitudinal plasma viral loads. The horizontal dashed line denotes the assay quantification limit (2.8 vRNA copies/ml plasma). Plot symbols below the quantification limit represent specimens in which no viral RNA was detected. (d) Longitudinal cell-associated viral DNA (CA-vDNA) levels in PBMC. Determined vDNA levels were normalized based on input diploid genome cell equivalents assayed for each sample determined by the duplex quantification of the copy number of a host cell gene within the same sample extraction. The horizontal dashed line indicates the maximum quantification limit across all analysed samples (15 vDNA copies/10⁶ cell equivalents). Plot symbols below the quantification limit represent specimens in which no vDNA was detected. Symbols are plotted at the threshold sensitivity limit for that individual sample based on the number of cell equivalents assayed.