Fig. 6.

GAPDH reduction inhibits cell proliferation and migration. (A) Western blotting and (B) statistical analysis show GAPDH protein expression in the lung cancer cell line H1299 and the breast cancer cell line MDA-MB231 transfected with GAPDH shRNAs. (C) Cell Counting Kit-8, (D and E) EdU proliferation and (F and G) propidium iodide staining assays show the inhibited cell proliferation ability of cells transfected with shRNAs. (H) Representative images and (I) statistical analysis of the results of Transwell cell migration assay showing the inhibited cell migration ability of cells transfected with shRNAs. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; shRNA, short hairpin RNA.