Fig. 7.

GAPDH is regulated by copy number amplification and DNA methylation. (A) DNA copy number variation analysis in 33 cancer types. (B) DNA methylation analysis in 33 cancer types. A red ID probe indicates that the probe targeted to the promotor region. (C) Methylation-specific PCR analysis of the GAPDH promoter in H1299, MDA-MB-231 and T24 cells. (D) mRNA and (E) protein expression of GAPDH in H1299, MDA-MB-231 and T24 cells treated with 5-aza-2'-deoxycytidine. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.