Figure 3.

ATGL-mediated lipolysis drives the production of a wide spectrum of bioactive lipid mediators during nutrient sufficiency and during serum starvation. (A–F) UPLC-MS-MS analysis of lipid mediators released from fed (A, B, E) and serum-starved (C, D, F) MDA-MB-231 cells pre-treated with hGX sPLA₂, and from ATGL-depleted cells without and with hGX sPLA₂ pre-treatment, presented as volcano plots (A, D), heat maps (B, C), and graphs for selected individual species (E, F). Volcano plots were prepared using log₂-transformed fold-change values and multiple t-test analysis, and the heat map by –logP data transformation and two-way ANOVA with Sidak adjustment (n = 3 independent experiments). (G) Diagram illustrating the involvement of hGX sPLA₂ and ATGL in the production of a wide range of PUFA-derived cyclooxygenase (COX) and lipoxygenase (LOX) signalling molecules. Data are means ± SEM of three independent experiments. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001 (two-way ANOVA with Sidak adjustment).