FIG. 1.
Representative patterns obtained following DRE-PCR analysis of M. tuberculosis clinical isolates. (A and B) Results for isolates that were clustered by neither IS6110-RFLP nor spoligotyping. (A) Lanes: A, molecular weight (MW) marker; B, negative control; C to I, various unclustered isolates (GP14, GP35, GP16, GP5, GP25, GP33, and GP10); J, MW marker. (B) Lanes: A, MW marker; B to H, various unclustered isolates (GP1, PMON, GP34, GP9, A11, GP39, and GP15); I, negative control; J, an unclustered isolate, GP29, that did not generate any bands upon DRE-PCR; K, isolate B4 from cluster B; L, unclustered isolate GP37; M, MW marker. (C) DRE-PCR analysis of isolates that were clustered by IS6110-RFLP analysis followed by spoligotyping. Lanes: A to E, five isolates from four patients of cluster B; F, an unclustered isolate (GP20); G, MW marker; H, an isolate of cluster C. DNA MW markers IX (A and B) and VI (C), from Boehringer Mannheim, Meylan, France, were used.