TABLE 1.
Molecular description of clusters observed for 72 M. tuberculosis clinical isolates from 52 patients by IS6110-RFLP and DR-RFLP analyses, followed by spoligotyping and DRE-PCR
Cluster | No. of IS6110-RFLP bands | No. of DR-RFLP bands (size [bp]) | Spoligotype | No. of major DRE-PCR bands (size [bp]) | No. of strainsa |
---|---|---|---|---|---|
A | 4–5 | 2 (4,800; 3,000) | ▪□▪□□□□□□□□□▪▪▪▪▪□▪▪▪▪▪▪▪▪▪▪▪▪▪▪□□□□▪▪▪▪▪▪▪ | 1 (180) | 12 |
B | 13 | 1 (5,100) | ▪▪▪▪▪□□□□□□□□□□□□▪▪□□□□□□□□□□□□□□□□□□□▪▪▪▪▪ | 1 (625) | 3 |
C | 9–11 | 2 (4,750; 3,000) | ▪□▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪□□□□□□□□▪▪▪▪□□□□▪▪▪▪▪▪▪ | 1 (500) | 2 |
The number of clustered isolates was based on IS6110-RFLP analysis followed by DR-RFLP analysis.